Glucocorticoid-regulated bidirectional enhancer RNA transcription pinpoints functional genetic variants linked to asthma

S.K. Sasse, A. Dahlin, L. Sanford, M.A. Gruca, A. Gupta, A. Chen Wu, C. Ibarren, R.D. Dowell, S.T. Weiss, A.N. Gerber. medRxiv (2022)

Extracellular matrix stiffness controls cardiac valve myofibroblast activation through epigenetic remodeling

C.J. Walker, D. Batan, C.T. Bishop, D. Ramirez, B.A. Aguado, M.E. Schroeder, C. Crocini, J. Schwisow, K. Moulton, L. Macdougall, R.M. Weiss, M.A. Allen, R. Dowell, L.A. Leinwand, K.S. Anseth. BioEngineering and Translational Medicine 7(3):e10394 (2022)

Integrated genomics approaches identify mediators of transcriptional and epigenetic responses to Afghan desert particulate matter in human small airway epithelial cells

A. Gupta, S.K. Sasse, R. Berman, M.A. Gruca, R.D. Dowell, H.W. Chu, G.P. Downey, A.N. Gerber. Physiological Genomics 54(10):389-401 (2022)

Protocol Variations in Run-On Transcription Dataset Preparation Produce Detectable Signatures in Sequencing Libraries

S. Hunter, R. Sigauke, J. Stanley, M. Allen, R.D. Dowell. BMC Genomics 23, 187 (2022)

Suppression of p53 response by targeting p53-Mediator binding with a stapled peptide

B.L. Allen*, K. Quach*, T. Jones*, C.B. Levandowski, C.C. Ebmeier, J.D. Rubin, T. Read, R.D. Dowell, A. Schepartz, D.J. Taatjes. Cell Rep. 39(1):110630 (2022)


Deconvolution of multiplexed transcriptional responses to wood smoke particles defines rapid aryl hydrocarbon receptor signaling dynamics

A. Gupta, S.K. Sasse, M.A. Gruca, L. Sanford, R.D. Dowell, A.N. Gerber. J. of Biol. Chemistry 297(4):101147 (2021)

Escape of hair follicle stem cells causes stem cell exhaustion during aging

C. Zhang, D. Wang, J. Wang, L. Wang, W. Qiu, T. Kume, R. Dowell, and R. Yi. Nature Aging 1,889-903 (2021)

Characterization of global gene expression, regulation of metal ions, and infection outcomes in immune competent 129S6 mouse macrophages

L.N. Janiszewski, M. Minson, M.A. Allen, R.D. Dowell, A.E. Palmer. Infect. Immun. (2021)

Chromatin remodeling due to degradation of citrate carrier impairs osteogenesis of aged mesenchymal stem cells

A. Pouikli, S. Parekh, M. Maleszewska, M. Baghdadi, I. Tripodi, C. Nikopoulou, K. Folz-Donahue, Y. Hinze, A. Mesaros, P. Giavalisco, R. Dowell, L. Partridge, and P. Tessarz. Nature Aging 1,810-825 (2021)

The ?40p53 isoform inhibits p53-dependent eRNA transcription and enables regulation by signal-specific transcription factors during p53 activation

C.B. Levandowski, T. Jones, M. Gruca, S. Ramamoorthy, R.D. Dowell#, D.J. Taatjes#. PLoS Biology 19(8): e3001364 (2021)

Myonuclear maturation dynamics in aged and adult regenerating mouse skeletal muscle.

J.V. Kurland, A.V. Deusen, B. Pawlikowski, M. Hall, N.D. Betta, T. Antwine, A.A. Cutler, A. Russell, M.A. Allen, R.D. Dowell, B.B. Olwin. bioRxiv (in review) (2021)

Application of a bioinformatic pipeline to RNA-seq data identifies novel viruslike sequence in human blood

M. Melnick, P. Gonzales, T.J. LaRocca, Y. Song, J. Wuu, M. Benatar, B. Oskarsson, L. Petrucelli, R.D. Dowell, C.D. Link, M. Prudencio. G3 11:9 (2021)

Just 2% of SARS-CoV-2-positive individuals carry 90% of the virus circulating in communities

Q. Yang, T.K. Saldi, E. Lasda, C.J. Decker, C.L. Paige, D. Muhlrad, P.K. Gonzales, M.R. Fink, K.L. Tat, C.R. Hager, J.C. Davis, C.D. Ozeroff, N.R. Meyerson, S.K. Clark, W.T. Fattor, A.R. Gilchrist, A. Barbachano-Guerrero, E.R. Worden-Sapper, S.S. Wu, G.R. Brisson, M.B. McQueen, R.D. Dowell, L. Leinwand, R. Parker, S.L. Sawyer. PNAS 118(21):e2104547118 (2021)

Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts

C.J. Walker, C. Crocini, D. Ramirez, A.R. Killaars, J.C. Grim, B.A. Aguado, S.K. Clark, M. Allen, R. Dowell, L.A. Leinwand*, K.S. Anseth*. Nature Biomedical Engineering 5, 1517-1518 (2021)

Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells

D.T. Youmans, A.R. Gooding, R.D. Dowell, T.R. Cech. Molecular Cell 81(3):p488-501.E9 (2021)

The MUC5B-associated variant rs35705950 resides within an enhancer subject to lineage- and disease-dependent epigenetic remodeling

F. Gally, S.K. Sasse, J.S. Kurche, M.A. Gruca, J.H. Cardwell, T. Okamoto, H.W. Chu, X. Hou, O.B. Poirion, J. Buchanan, S. Preissl, B. Ren, S.P. Colgan, R.D. Dowell, I.V. Yang, D.A. Schwartz, A.N. Gerber. JCI Insight 6(2) (2021)

Transcription factor enrichment analysis (TFEA) quantifies the activity of multiple transcription factors from a single experiment.

J.D. Rubin, J.T. Stanley, R.F. Sigauke, C.B. Levandowski, Z.L. Maas, J. Westfall, D.J. Taatjes & R.D. Dowell. Communications Biology 4:661 (2021)


Systems genetics analysis of the LXS recombinant inbred mouse strains: Genetic and molecular insights into acute ethanol tolerance

R.A. Radcliffe , R. Dowell, A.T. Odell, P.A. Richmond, B. Bennett, C. Larson, K. Kechris, L.M. Saba, P. Rudra, S. Wen. PLoS ONE 15(10): e0240253 (2020)

Selective inhibition of CDK7 reveals high-confidence targets and new models for TFIIH function in transcription

J.K. Rimel, Z.C. Poss, B. Erickson, Z.L. Maas, C.C. Ebmeier, J.L. Johnson, T-M. Decker, T.M. Yaron, M.J. Bradley, K.B. Hamman, S. Hu, G. Malojcic, J.J. Marineau, P.W. White, M. Brault, L. Tao, P. DeRoy, C. Clavette, S. Nayak, L.J. Damon, I.H. Kaltheuner, H. Bunch, L.C. Cantley, M. Geyer, J. Iwasa, R.D. Dowell, D.L. Bentley, W.M. Old and D.J. Taatjes. Genes and Development 34: 1452-1473 (2020)

Applying knowledge-driven mechanistic inference to toxicogenomics

I.J. Tripodi, T. Callahan, J. Westfall, N. Meitzer, R. Dowell, L. Hunter. Toxicology in Vitro 66:104877 (2020)

Combining signal and sequence to detect RNA polymerase initiation in ATAC-seq data

I.J. Tripodi, M. Chowdhury, M. Gruca, R.D. Dowell. PLoS ONE 15(4):e0232332 (2020)

TFIID enables RNA Polymerase II promoter-proximal pausing

C.B. Fant, C.B. Levandowski, K. Gupta, Z.L. Maas, J. Moir, J.D. Rubin, A. Sawyer, M.N. Esbin, J.K. Rimel, O. Luyties, M.T. Marr, I. Berger, R.D. Dowell, D.J. Taatjes. Molecular Cell 78(4):785-793 (2020)

Remodeling of Zn2+ homeostasis upon differentiation of mammary epithelial cells

Y. Han, L. Sanford, D.M. Simpson, R.D. Dowell, A.E. Palmer. Metallomics 12:346-362 (2020)

Lessons from eRNAs: understanding transcriptional regulation through the lens of nascent RNAs.

J.F. Cardiello, G.J. Sanchez, M.A. Allen, R.D. Dowell. Transcription 11(1):3-18 (2020)


Two-stage ML Classifier for Identifying Host Protein Targets of the Dengue Protease

J.T. Stanley, A.R. Gilchrist, A.C. Stabell, M.A. Allen, S.L. Sawyer, and R.D. Dowell. Pac Symp Biocomputing 2020 Dec 2019, 487-498 (2019)

Annotation Agnostic Approaches to Nascent Transcription Analysis: Fast Read Stitcher and Transcription Fit

M.A. Gruca, M.A. Gohde, R.D. Dowell. Methods in Molecular Biology (2019)

Nascent transcript analysis of glucocorticoid crosstalk with TNF defines primary and cooperative inflammatory repression

S.K. Sasse, M. Gruca, M.A. Allen, V. Kadiyala, T. Song, F. Gally, A. Gupta, M.A. Pufall, R.D. Dowell, A.N. Gerber. Genome Research 29:1753-1765 (2019)

Transcriptional Responses to INFgamma require mediator kinase-dependent pause release and mechanistically distinct CDK8 and CDK19 functions

I. Steinparzer, V. Sedlyarov, J.D. Rubin, K. Eislmayr, M.D. Galbraith, C.B. Levandowski, T. Vcelkova, L. Sneezum, F. Wascher, F. Amman, R. Kleinova, J.M. Espinosa, G. Superti-Furga, R.D. Dowell, D.J. Taatjes*, P. Kovarik*. Molecular cell 76(3):485-499 (2019)

Identification and characterization of a novel anti-inflammatory lipid isolated from Mycobacterium vaccae, a soil-derived bacterium with immunoregulatory and stress resilience properties

D.G. Smith, R. Martinelli, G.S. Besra, P.A. Illarionov, I. Szatmari, P. Brazda, M.A. Allen, W. Xu, X. Wang, L. Nagy, R.D. Dowell, G.A. W. Rook, L.R. Brunet, C.A. Lowry. Psychopharmacology May;236(5):1653-1670 (2019)

Heat shock in C. elegans induces downstream of gene transcription and accumulation of double-stranded RNA

M. Melnick, P. Gonzales, J. Cabral, M.A. Allen, R.D. Dowell, C.D. Link. PLoS ONE Apr 8;14(4):e0206715 (2019)

Train students to navigate ethical swamps

M.A. Allen. Nature 568, 145 (2019)


Control of inducible gene expression links cohesin to hematopoietic progenitor self-renewal and differentiation.

S. Cuartero, F.D. Weiss, G. Dharmalingam, Y. Guo, E. Ing-Simmons, S. Masella, I. Robles-Rebollo, X. Xiao, Y.F. Wang, I. Barozzi, D. Djeghloul, M.T. Amano, H. Niskanen, E. Petretto, R.D. Dowell, K. Tachibana, M.U. Kaikkonen, K.A. Nasmyth, B. Lenhard, G. Natoli, A.G. Fisher, and M. Merkenschlager. Nature Immunology Sept 19(9):932-941 (2018)

PI(3,5)P2 Controls Vacuole Potassium Transport to Support Cellular Osmoregulation

Z.N. Wilson, A.L. Scott, R.D. Dowell, and G. Odorizzi. Molecular Biology of the Cell 29(14):1718-1731 (2018)

Detecting Differential Transcription Factor Activity from ATAC-Seq Data

I.J. Tripodi, M.A. Allen, and R.D. Dowell. Molecules 23(5), 1136 (2018)

miR-MaGiC improves quantification accuracy for small RNA-seq

P.H. Russell, B. Vestal, W. Shi, P.D. Rudra, R. Dowell, R. Radcliffe, L. Saba and K. Kechris. BMC Research Notes 11:296 (2018)

Enhancer RNA profiling predicts transcription factor activity

J.G Azofeifa, M.A. Allen, J.R. Hendrix, T. Read, J.D. Rubin, and R.D. Dowell. Genome Research 28: 334-344 (2018)

Genome-wide dose-dependent inhibition of histone deacetylases studies reveal their roles in enhancer remodeling and suppression of oncogenic super-enhancers

G.J. Sanchez, P.A. Richmond, E.N. Bunker, S.S. Karman, J. Azofeifa, A.T. Garnett, Q. Xu, G.E. Wheeler, C.M. Toomey, Q. Zhang, R.D. Dowell and X. Liu. Nucleic Acids Research 46(4):1756-1776 (2018)

Earlier Publications

A communal catalogue reveals Earth’s multiscale microbial diversity
L.R. Thompson, J.G. Sanders, D. McDonald, A. Amir, J. Ladau, K.J. Locey, ..(et. al.) .. and The Earth Microbiome Project Consortium.
Nature 551(7681):457-463 (2017)

A kinase-independent role for CDK19 in p53 response
K.A. Audetat, M.D. Galbraith, A.T. Odell, T. Lee, A. Pandey, J.M. Espinosa, R.D. Dowell and D.J. Taatjes.
Molecular and Cellular Biology 37:13 (2017)

Discriminating between HuR and TTP binding sites using the k-spectrum kernel method.
S. Bhandare, D.S. Goldberg, and R.D. Dowell.
PLoS ONE 12(3):e0174052 (2017)

Transcriptome and Functional Profile of Cardiac Myocytes Is Influenced by Biological Sex
C.L. Trexler, A.T. Odell, M.Y. Jeong, R.D. Dowell, L.A. Leinwand.
Circulation: Cardiovascular Genetics 10:e001770 (2017)

Human TFIIH kinase CDK7 regulates transcription-associated epigenetic modifications
C.C. Ebmeier, B. Erickson, B.L. Allen, M.A. Allen, H. Kim, N. Fong, J.R. Jacobsen, K. Liang, A. Shilatifard, R.D. Dowell, W.M. Old, D.L. Bentley, and D.J. Taatjes.
Cell Reports 20(5):1173-1186 (2017)

The Influence of Polyploidy on the Evolution of Yeast Grown in a Sub-Optimal Carbon Source
A.L. Scott, P.A. Richmond, R.D. Dowell, A.M. Selmecki.
Molecular Biology and Evolution 34(10):2690-2703 (2017)

A generative model for the behavior of RNA polymerase
J.G. Azofeifa and R.D. Dowell.
Bioinformatics 33(2):227-234 (2017)

Model based heritability scores for high-throughput sequencing data
P. Rudra, W. J. Shi, B. Vestal, P.H. Russell, A. Odell, R.D. Dowell, R.A. Radcliffe, L.M. Saba and K. Kechris.
BMC Bioinformatics 18:143 (2017)

RNA Pol II transcription model and interpretation of GRO-seq data
M.E. Lladser, J.G. Azofeifa, M.A. Allen, R.D. Dowell.
Journal of Mathematical Biology 74:77 (2017)

An annotation agnostic algorithm for detecting nascent RNA transcripts in GRO-seq
J. Azofeifa, M.A. Allen, M.Lladser, and R.D. Dowell.
IEEE/ACM Transactions on Computational Biology and Bioinformatics 14(5):1070-1081 (2017)

Genome Characterization of the Selected Long and Short Sleep Mouse Lines
R.D. Dowell, A. Odell, P. Richmond, D. Malmer, E. Halper-Stromberg, B. Bennett, C. Larson, S. Leach, R.A. Radcliffe.
Mammalian Genome 27:574 (2016)

Survey of cryptic unstable transcripts in yeast
J. Vera and R.D. Dowell.
BMC Genomics 17:305 (2016)

Evidence for Association Between Low Frequency Variants in CHRNA6/CHRNB3 and Antisocial Drug Dependence
H.M. Kamens, R.P. Corley, P.A. Richmond, T.M. Darlington, R.Dowell, C.J. Hopfer, M.C. Stallings, J.K. Hewitt, S.A. Brown, M.A. Ehringer.
Behavior Genetics Sep;46(5):693-704 (2016)

Identification of Mediator kinase substrates in human cells using cortistatin A and quantitative phosphoproteomics.
Z.C. Poss, C.C. Ebmeier, A. Odell, A. Tangpeerachaikul, T. Lee, H. E. Pelish, M.D. Shair, R.D. Dowell, W. Old, D.J. Taatjes.
Cell Reports Apr 12;15(2):436-50 (2016)

Foxc1 reinforces quiescence in self-renewing hair follicle stem cells.
L. Wang, J.A. Siegenthaler, R.D. Dowell, R. Yi.
Science 351(6273): 613-617 (2016)

A trans-acting variant within the transcription factor RIM101 interacts with genetic background to determine its regulatory capacity
T. Read, P.A. Richmond, and R.D. Dowell.
PLoS Genetics 12(1): e1005746 (2016)

A modeling framework for generation of positional and temporal simulations of transcriptional regulation
D.A. Knox and R.D. Dowell.
IEEE/ACM Transactions on Computational Biology and Bioinformatics May-June 13(3):459-71; ISSN: 1545-5963 (2016)

Prolonged Cre expression driven by the ?-myosin heavy chain promoter can be cardiotoxic
E.K. Pugach, P.A. Richmond, J.G. Azofeifa, R.D. Dowell, L.A. Leinwand.
Journal of Molecular and Cellular Cardiology 86:54-61 (2015)

Polyploidy can drive rapid adaptation in yeast
A. Selmecki, Y.E. Maruvka, P.A. Richmond, M. Gullet, N. Shoresh, A. Sorenson, S. De, R. Kishony, F. Michor, R.D. Dowell, D. Pellman.
Nature 519, 349–352 (2015)

QTL mapping of acute functional tolerance in the LXS recombinant inbred strains
B. Bennett, C. Larson, P.A. Richmond, A. Odell, L. Saba, B. Tabakoff, R.D. Dowell, R. Radcliffe.
Alcoholism: Clinical and Experimental Research 39(4):611-620 (2015)

An Engineered Calcium-Precipitable Restriction Enzyme
J. Hendrix, T. Read, J-F. Lalonde, P.K Jensen, W. Heymann, E.Z. Lovelace, S.A. Zimmermann, M. Brasino, J. Rokicki, R.D. Dowell.
ACS Synthetic Biology 3(12):969-971 (2014)

Global analysis of p53-regulated transcription identifies its direct targets and unexpected regulatory mechanisms
M.A. Allen, Z. Andrysik, V.L. Dengler, H.S. Mellert, A. Guarnieri, J.A. Freeman, K.D. Sullivan, M.D. Galbraith, X. Luo, W.L. Kraus, R.D. Dowell, and J.M. Espinosa.
eLife 2014;3:e02200 (2014)

CodaChrome: a tool for the visualization of proteome conservation across all fully sequenced bacterial genomes
J. Rokicki, D. Knox, R.D. Dowell, and S.D. Copley.
BMC Genomics 15:65 (2014)

FStitch: A fast and simple algorithm for detecting nascent RNA transcripts
J. Azofeifa, M.A. Allen, M.Lladser, and R.D. Dowell.
5th ACM Conference on Bioinformatics, Computational Biology and Health Informatics Sept. (2014)

Retrospective reflections of a whistleblower - Opinions on misconduct responses
M.A. Allen and R.D. Dowell.
Accountability in Research 20:5-6, 339-348 (2013)

HIF1A employs CDK8-Mediator to stimulate RNAPII elongation in response to hypoxia
M.D. Galbraith, M.A. Allen, C.L. Bensard, X. Wang, M.K. Schwinn, B. Qin, H.W. Long, D.L. Daniels, W.C. Hahn, R.D. Dowell and J.M. Espinosa.
Cell 153(6) pp. 1327 - 1339 (2013)

Genome-wide maps of polyadenylation reveal dynamic mRNA 3?-end formation in mammalian cell lineages
L. Wang, R.D. Dowell and R. Yi.
RNA 19(3):413-425 (2013)

sizzled function and secreted factor network dynamics
J. Shi, H. Zhang, R.D. Dowell and M.W. Klymkowsky.
Biology Open 1, 286-294 (2012)

Discovering Regulatory Overlapping RNA Transcripts
T. Danford, R. Dowell, S. Agarwala, P. Grisafi, G. Fink, D. Gifford.
Journal of Computational Biology 18(3): 295-303 (2011)

The similarity of gene expression between human and mouse tissues
R.D. Dowell.
Research Highlight - Genome Biology 12:101 (2011)

Transcription factor binding variation in the evolution of gene regulation
R.D. Dowell.
Trends in Genetics 26(11):468-475 (2010)

Regulatory Overlapping RNA Transcripts
T. Danford, R. Dowell, S. Agarwala, P. Grisafi, G. Fink, D. Gifford.
14th Annual International Conference, RECOMB LNBI 6044:110-122 (2010)

Feed-forward Regulation of a Cell Fate Determinant by an RNA-binding Protein Generates Asymmetry in Yeast
J.J. Wolf, R.D. Dowell, S. Mahony, M. Rabani, D.K. Gifford, G.R. Fink.
Genetics 185:513-522 (2010)

Genotype to Phenotype: A Complex Problem
R.D. Dowell*, O. Ryan*, A. Jansen, D. Cheung, S. Agarwala, T. Danford, D.A. Bernstein, P.A. Rolfe, L.E. Heisler,B. Chin, C. Nislow, G. Giaever, P.C. Phillips, G.R. Fink, D.K. Gifford, C. Boone.
Science 328(5977):469 (2010)

Toggle involving cis-interfering RNAs controls variegated gene expression in yeast
S.L. Bumgarner, R.D. Dowell, P. Grisafi, D.K. Gifford, G.R. Fink.
PNAS 106(43):18321-18326 (2009)

Automated discovery of functional generality of human gene expression programs
G.K. Gerber, R.D. Dowell, T.S. Jaakkola, and D.K. Gifford.
PLoS Computational Biology 3(8):e148 (2007)

Tissue-specific transcriptional regulation has diverged significantly between human and mouse
D.T. Odom, R.D. Dowell, E.S. Jacobsen, W. Gordon, T.W. Danford, K.D. MacIsaac, P.A. Rolfe, C.M. Conboy, D.K. Gifford, and E. Fraenkel.
Nature Genetics 39(6):730-2. (2007)

Efficient pairwise RNA structure prediction and alignment using sequence alignment constraints
R.D. Dowell and S.R. Eddy.
BMC Bioinformatics 7:400. (2006)

Core transcriptional regulatory circuitry in human hepatocytes
D.T. Odom, R.D. Dowell, E.S. Jacobsen, L. Nekludova, P.A. Rolfe, T.W. Danford, D.K. Gifford, E. Fraenkel, G.I. Bell, and R.A. Young.
Molecular Systems Biology 2:2006.0017. (2006)

High-resolution computational models of genome binding events
Y. Qi, P.A. Rolfe, K.D. MacIsaac, G.K. Gerber, D. Pokholok, J. Zeitlinger, T.W. Danford, R.D. Dowell, E. Fraenkel, T.S. Jaakkola, R.A. Young, and D.K. Gifford.
Nature Biotechnology 24(8):963-70. (2006)

Evaluation of several lightweight stochastic context-free grammars for RNA secondary structure prediction
R.D. Dowell and S.R. Eddy.
BMC Bioinformatics 5:71. (2004)

The distributed annotation system
R.D. Dowell, R.M. Jokerst, A. Day, S.R. Eddy, and L. Stein.
BMC Bioinformatics 2:7(2001)